Workshop on "Cell Biology, Fluorescence Microscopy, and Geometry"

Poster

Linda Cheung  (University of Warwick, United Kingdom)

MELK analysis of tumour cell dormancy

L.Cheung1, S. Pelengaris1, S. Abouna1, V. Ifandi1, R. Koop3, D. Epstein2, W. Schubert3 and M. Khan1 1Biomedical Research Institute, Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK, 2 Department of Mathematics, University of Warwick, Coventry, CV4 7AL, UK, and 3MelTec GmbH & Co. KG, Zenit-Building, Magdeburg, Germany.



We have employed a 'switchable' transgenic mice (pIns-MycERTAM), developed by our group, to explore the role of the c-Myc oncoprotein in cellular apoptosis, proliferation and transformation in vivo. The advantage in using this model is that c-Myc activity in β-cells can be carefully controlled and is switched 'on' by 4-hydroxytamoxifen (4-OHT), and is switched 'off' by withdrawing 4-OHT.

Our earlier work has identified c-Myc-induced apoptosis as a major inherent tumour suppressor activity of c-Myc (1). Moreover if c-Myc apoptosis is prevented by over-expressing Bcl-xL, c-Myc is sufficient to induce all the hallmark features of cancer (unrestrained replication, loss of differentiation, angiogenesis and invasion). Importantly, tumourigenesis continues to require c-Myc activation and inactivation of c-Myc can fully regress tumours.

We are currently looking at changes in c-Myc regulated gene and protein expression using microarrray and proteomics. However, these methods will not uncover the key interactions between c-Myc activated cancer cells and other cells in the tumour microenvironment. Changes in gene expression can be localised to individual cells by laser capture and RNA isolation but single cell techniques for gene expression remain problematic and anatomical context is lost. MELK (Multi-Epitope-Ligand-Kartographie), a form of in situ proteomics, can overcome this limitation as it enables multiple proteins (over 100) to be examined on the same tissue section without disrupting it first.

A recent study by Jain et al., showed that brief inactivation (10 days) of c-Myc was sufficient for the sustained regression of c-Myc induced invasive osteogenic sarcomas in transgenic mice; subsequent re-activation of c-Myc led to extensive apoptosis as opposed to restoration of the neoplastic phenotype (2). Here, and in a recent paper that we have published, we show that inactivating c-Myc transiently for 9 days in islet tumours does not result in accelerated apoptosis but instead restores the oncogenic properties of c-Myc (3).

1. Pelengaris S, Khan M, Evan G: Suppression of Myc-induced apoptosis in beta cells exposes multiple oncogenic prperties of Myc and triggers carcinogenic progression. Cell 2002, 109:321-334.

2. Jain M, Arvanitis C, Chu K, Dewey W, Leonhardt E, Trinh M, Sundberg CD, Bishop JM, Felsher DW: Sustained loss of a neoplstic phenotype by brief inactivation of MYC. Science 2002, 297:102-104.

3. Pelengaris S, Abouna S, Cheung L, Ifandi V, Zervou S, Khan M: Brief inactivation of c-Myc is not sufficient for sustained regression of c-Myc-induced tumours of pancreatic islets and skin epidermis. BMC Biology 2004, 2:26.
Ulrich Randoll  (Matrix-Center-München, Germany)

Matrix-Rhythm-Therapy in Osteopathic thought.

U.G. Randoll1,2,3, B. Dickreiter3,4, F.F. Hennig2
1 Matrix-Center München, Plinganser Str. 45; 81369 München
2 Dep. of Traumatology, Univ. Erlangen-Nuremberg, 91054 Erlangen
3 Matrix-Center-Reha-Klinik Klausenbach; LVA Baden Württemberg; 77787 Nordrach
4 Thomas-Wildey-Institut, Munich
URL: www.matrix-center.com
e-mail: info@matrix-center.com

In the field of osteopathy cranial and vasomotor rhythms are well known. Also the meaning of their changes until a total stop have been described for decades.
Coming from the practical treatment and experience side, osteopathy uses till now empirically found body-vibrations for diagnostics as well as therapy.
Biophysics of today shows clearly how biological structures are the result of physico-chemical processes that are driven by body intrinsic and / or body external rhythms, and the whole span of life in the sense of bio-informative fields.
Following this dynamic approach at the beginning of the 90's, researchers in the department of traumatology at the University of Erlangen-Nuremberg developed the Matrix-Rhythm-Therapy, a 'deep working rhythmic Micro-Extension-Technique' that is for the first time presented to osteopaths.
The researchers from Erlangen started to find out how far time patterns are disturbed in diseases and how far they can be systematically and continuously restored and brought back to a synchronous cooperation up to the macroscopic level again by using the actual ideas and findings of cellular biophysics.
The therapy orients itself at the so far neglected time-structure (time-pattern) of the organism and can be synergistically used in present day modern Osteopathy

Date and Location

June 05 - 08, 2005
Max Planck Institute for Mathematics in the Sciences
Inselstraße 22
04103 Leipzig
Germany
see travel instructions

Scientific Organizers

Andreas Dress
Max Planck Institute for Mathematics in the Sciences
Leipzig
Contact by Email

Jean-Pierre Bourguignon
Institut des Hautes Études Scientifiques
Bures-sur-Yvette

Administrative Contact

Antje Vandenberg
Max Planck Institute for Mathematics in the Sciences
Leipzig
Contact by Email

05.04.2017, 12:42